Summary
Type: SPE
Application #: 304530
Catalog#: 730079 - SPE Cartridges, Chromabond, SB, 3ml, PP, 500mg, 50/pk
Detection: HPLC or GC
Conditions: Column conditioning: 3 ml methanol, then 3 ml water.
(Do not let run the column dry.)
Sample aspiration: The prepared sample is passed through the column by vacuum or pressure (max. 1000 ml sample volume)
Washing 1: With 3 ml 5% NH3 in water acidic impurities are removed.
Washing 2 (Elution 1): With 3 ml methanol / water (20:80, v/v) neutral and basic compounds can be removed (eluated).
Elution 2: Compounds are eluted with 2 x 2 ml methanol/acetone (1:1, v/v) + 5% AcOH or with 2 x 2 ml methanol/acetone (1:1, v/v) + 20% NH3
Authors: SPE department
Source: Macherey-Nagel